Rapid diagnosis of poliovirus infection by PCR amplification

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Rapid diagnosis of poliovirus infection by PCR amplification.

A single-tube, single-primer-set reverse transcription-PCR assay was developed for the rapid detection of polioviruses in infected tissue culture fluids and clinical materials. The poliovirus-specific PCR primers are located in the VP1-2A region of the poliovirus genome. They generate a 290-bp product and can be used in duplex reactions with general enterovirus primers. The primers span the reg...

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BACKGROUND Traditionally, the most common diagnostic approach used for diagnosing leptospirosis was the demonstration of immune-seroconversion in acute and convalescent patient serum samples. Recently, a variety of molecular techniques, including conventional and real-time polymerase chain reaction (PCR), have been developed for the specific detection of pathogenic bacteria from the genus Lepto...

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Rapid Diagnosis by PCR in Children

Background The diagnosis of viral myocarditis remains difficult and generally depends on clinical and histological criteria. Viral cultures and serology are often unrewarding, with low yields. The purpose of this study was to analyze the usefulness of polymerase chain reaction (PCR) in the rapid diagnosis of acute myocarditis in children. Methods and Results PCR was used to analyze 38 myocardia...

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rapid diagnosis of neisseria meningitides by pcr method

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1996

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.34.7.1722-1725.1996